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CUL7 Monoclonal antibody

CUL7 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E3G9

Cat no : 67034-1-Ig

Synonyms

CUL 7, CUL7, cullin 7, dJ20C7.5, KIAA0076

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of HEK-293 using 67034-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of HEK-293 using 67034-1-Ig

    WB result of CUL7 antibody (67034-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HSC-T6 cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of HSC-T6 using 67034-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of HeLa using 67034-1-Ig

    WB result of CUL7 antibody (67034-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HeLa cells.

  • Western Blot (WB) analysis of NCI-H1299 cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of NCI-H1299 using 67034-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL7 Monoclonal antibody (67034-1-Ig)

    IHC staining of human heart using 67034-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL7 Monoclonal antibody (67034-1-Ig)

    IHC staining of human heart using 67034-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NCI-H1299 cells, HSC-T6 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67034-1-Ig targets CUL7 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CUL7 fusion protein Ag6943 相同性解析による交差性が予測される生物種
Full Name cullin 7
Calculated molecular weight 1698 aa, 191 kDa
Observed molecular weight 185 kDa
GenBank accession numberBC033647
Gene symbol CUL7
Gene ID (NCBI) 9820
RRIDAB_2882349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

he cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. CUL7 was originally discovered as 185-kDa protein associated with the large T antigen of simian virus 40 (SV40). CUL7-deficient mice exhibit neonatal lethality with reduced size and vascular defects. CUL7 presumably plays a role in the DNA damage response by limiting p53 activity. CUL7 mutations have also been identified in 3-Msyndrome and the Yakuts short stature syndrome, both of which are characterized by pre- and post-natal growth retardation but with relatively normal mental and endocrine functions, suggesting that CUL7 may also be crucial for human placental development.

Protocols

Product Specific Protocols
WB protocol for CUL7 antibody 67034-1-IgDownload protocol
IHC protocol for CUL7 antibody 67034-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols