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  • Featured Product
  • KD/KO Validated

CUL7 Monoclonal antibody, PBS Only

CUL7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E3G9

Cat no : 67034-1-PBS

Synonyms

CUL 7, CUL7, cullin 7, dJ20C7.5, KIAA0076

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    WB result of CUL7 antibody (67034-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HEK-293 cells. This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of HSC-T6 using 67034-1-Ig (same clone as 67034-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of HeLa using 67034-1-Ig (same clone as 67034-1-PBS)

    WB result of CUL7 antibody (67034-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HeLa cells. This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCI-H1299 cells using CUL7 Monoclonal antibody (67034-1-Ig)

    WB analysis of NCI-H1299 using 67034-1-Ig (same clone as 67034-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL7 Monoclonal antibody (67034-1-Ig)

    IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL7 Monoclonal antibody (67034-1-Ig)

    IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67034-1-PBS targets CUL7 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CUL7 fusion protein Ag6943 相同性解析による交差性が予測される生物種
Full Name cullin 7
Calculated molecular weight 1698 aa, 191 kDa
Observed molecular weight 185 kDa
GenBank accession numberBC033647
Gene symbol CUL7
Gene ID (NCBI) 9820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

he cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. CUL7 was originally discovered as 185-kDa protein associated with the large T antigen of simian virus 40 (SV40). CUL7-deficient mice exhibit neonatal lethality with reduced size and vascular defects. CUL7 presumably plays a role in the DNA damage response by limiting p53 activity. CUL7 mutations have also been identified in 3-Msyndrome and the Yakuts short stature syndrome, both of which are characterized by pre- and post-natal growth retardation but with relatively normal mental and endocrine functions, suggesting that CUL7 may also be crucial for human placental development.