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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CXCL9/MIG Recombinant antibody

CXCL9/MIG Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241437F12

Cat no : 84427-4-RR

Synonyms

MIG; CXCL9, MIG, CXCL9, C-X-C motif chemokine 9, CMK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCL9/MIG Recombinant antibody (84427-4-RR)

    WB analysis using 84427-4-RR

    IFN gamma and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 84427-4-RR (CXCL9 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CXCL9/MIG Recombinant antibody (84427-4-RR)

    WB analysis using 84427-4-RR

    IFN gamma treated THP-1 cells were subjected to SDS PAGE followed by western blot with 84427-4-RR (CXCL9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inLPS and IFN gamma treated THP-1 cells, IFN gamma treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84427-4-RR is CXCL9/MIG Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CXCL9/MIG fusion protein Ag17932 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) ligand 9
Calculated molecular weight 14 kDa
Observed molecular weight14 kDa
GenBank accession numberBC095396
Gene symbol CXCL9
Gene ID (NCBI) 4283
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCL9 is a small cytokine known as MIG. CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. A high level of CXCL9 revealed in peripheral liquids, can be considered as a marker of host immune response, especially of that involving Th1 cells.

Protocols

Product Specific Protocols
WB protocol for CXCL9/MIG antibody 84427-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols