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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CXCR3B-specific Monoclonal antibody

CXCR3B-specific Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2D6

Cat no : CL488-60065

Synonyms

CXCR3, CMKAR3, CKR-L2, CKR L2, CD183

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U-937 cells using CoraLite® Plus 488-conjugated CXCR3B-specific Mono (CL488-60065)

    IF Staining of U-937 using CL488-60065

    Immunofluorescent analysis of un-fixed Live U-937 cells using CoraLite® Plus 488 CXCR3B-specific antibody (CL488-60065, Clone: 1B2D6 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inLive U-937 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60065 targets CXCR3B-specific in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) receptor 3
Calculated molecular weight 46 kDa
Observed molecular weight 40 kDa+43 kDa
GenBank accession numberAF469635
Gene symbol CXCR3
Gene ID (NCBI) 2833
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CXCR3B-specific antibody CL488-60065Download protocol
Standard Protocols
Click here to view our Standard Protocols