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- KD/KO Validated
CXCR4 Monoclonal antibody
CXCR4 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgM
Reactivity
human and More (1)
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
4B5E4
Cat no : 60042-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, THP-1 cells, Ramos cells, Daudi cells, Jurkat cells, Raji cells, K-562 cells |
Positive IHC detected in | human tonsillitis tissue, human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 8 publications below |
WB | See 38 publications below |
IHC | See 10 publications below |
IF | See 11 publications below |
FC | See 1 publications below |
CoIP | See 2 publications below |
Product Information
60042-1-Ig targets CXCR4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Immunogen | CXCR4 fusion protein Ag1528 相同性解析による交差性が予測される生物種 |
Full Name | chemokine (C-X-C motif) receptor 4 |
Calculated molecular weight | 352 aa, 40 kDa |
Observed molecular weight | 60~70 kDa |
GenBank accession number | BC020968 |
Gene symbol | CXCR4 |
Gene ID (NCBI) | 7852 |
RRID | AB_2091809 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).
Protocols
Product Specific Protocols | |
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WB protocol for CXCR4 antibody 60042-1-Ig | Download protocol |
IHC protocol for CXCR4 antibody 60042-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun HIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. | ||
J Exp Med CXCR4 regulates Plasmodium development in mouse and human hepatocytes.
| ||
Cancer Lett YTHDF1 promotes the osteolytic bone metastasis of breast cancer via inducing EZH2 and CDH11 translation | ||
Nanoscale Mitomycin C-treated human-induced pluripotent stem cells as a safe delivery system of gold nanorods for targeted photothermal therapy of gastric cancer | ||
ACS Appl Mater Interfaces Surface Functionalization of Polymeric Nanoparticles with Umbilical Cord-Derived Mesenchymal Stem Cell Membrane for Tumor-Targeted Therapy. |