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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCR4 Monoclonal antibody (60042-1-Ig)

    WB analysis using 60042-1-Ig (same clone as 60042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using CXCR4 Monoclonal antibody (60042-1-Ig)

    WB analysis of Daudi using 60042-1-Ig (same clone as 60042-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CXCR4 Monoclonal antibody (60042-1-Ig)

    WB analysis of THP-1 using 60042-1-Ig (same clone as 60042-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using CXCR4 Monoclonal antibody (60042-1-Ig)

    WB analysis of Ramos using 60042-1-Ig (same clone as 60042-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CXCR4 Monoclonal antibody (60042-1-Ig)

    IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CXCR4 Monoclonal antibody (60042-1-Ig)

    IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CXCR4 Monoclonal antibody (60042-1-Ig)

    IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXCR4 Monoclonal antibody (60042-1-Ig)

    IHC staining of human breast cancer using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXCR4 Monoclonal antibody (60042-1-Ig)

    IHC staining of human breast cancer using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CXCR4 Monoclonal antibody (60042-1-Ig)

    IHC staining of human prostate cancer using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CXCR4 Monoclonal antibody (60042-1-Ig)

    IHC staining of human prostate cancer using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CXCR4 Monoclonal antibody, PBS Only

CXCR4 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60042-1-PBS
Clone No.4B5E4

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CXCR-4, CXC-R4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60042-1-PBS targets CXCR4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen CXCR4 fusion protein Ag1528 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) receptor 4
Calculated molecular weight 352 aa, 40 kDa
Observed molecular weight 60~70 kDa
GenBank accession numberBC020968
Gene Symbol CXCR4
Gene ID (NCBI) 7852
RRIDAB_2091809
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP61073
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).