CXCR7 Monoclonal antibody
CXCR7 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
4C3D7
Cat no : 60216-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, U-87 MG cells, Jurkat cells, Raji cells, K-562 cells, THP-1 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human breast cancer tissue, human prostate cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 1 publications below |
Product Information
60216-1-Ig targets CXCR7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | CXCR7 fusion protein Ag14247 相同性解析による交差性が予測される生物種 |
Full Name | chemokine (C-X-C motif) receptor 7 |
Calculated molecular weight | 362 aa, 41 kDa |
Observed molecular weight | 50-60 kDa |
GenBank accession number | BC036661 |
Gene symbol | CXCR7 |
Gene ID (NCBI) | 57007 |
RRID | AB_11182925 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as a coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).
Protocols
Product Specific Protocols | |
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WB protocol for CXCR7 antibody 60216-1-Ig | Download protocol |
IHC protocol for CXCR7 antibody 60216-1-Ig | Download protocol |
IF protocol for CXCR7 antibody 60216-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Urol Oncol The inflammation-related biomarker CXCR7 independently predicts patient outcome after radical prostatectomy. |