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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SW480 cells using CoraLite® Plus 488-conjugated CXCR7 Monoclonal ant (CL488-60216)

    IF Staining of SW480 using CL488-60216

    Immunofluorescent analysis of (-20°C Methanol) fixed SW480 cells using CoraLite® Plus 488 CXCR7 antibody (CL488-60216, Clone: 4C3D7 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated CXCR7 Monoclonal antibody

CXCR7 Monoclonal Antibody for IF/ICC
Cat No. CL488-60216
Clone No.4C3D7

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

ACKR3, Chemokine orphan receptor 1, CMKOR1, CXC R7, CXCR 7, CXCR7, G protein coupled receptor 159, GPR159, RDC 1, RDC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inSW480 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60216 targets CXCR7 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CXCR7 fusion protein Ag14247 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) receptor 7
Calculated molecular weight 362 aa, 41 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC036661
Gene Symbol CXCR7
Gene ID (NCBI) 57007
RRIDAB_3672862
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25106
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CXCR7 antibody CL488-60216Download protocol
Standard Protocols
Click here to view our Standard Protocols