Featured Product
KD/KO Validated

CYB5R3 Polyclonal antibody

Name: CYB5R3 Polyclonal antibody
Brand: Proteintech
SKU: 10894-1-AP

CYB5R3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10894-1-AP

Synonyms

NADH-cytochrome b5 reductase 3, EC:1.6.2.2, Diaphorase-1, Diaphorase 1, DIA1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 10894-1-AP
WB results of CYB5R3 antibody (10894-1-AP, 1:4000) with si-Control and si-CYB5R3 transfected HeLa cells.
WB analysis using 10894-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10894-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 10894-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10894-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10894-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10894-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 10894-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 10894-1-AP
IP result of anti-CYB5R3 (IP:10894-1-AP, 3ug; Detection:10894-1-AP 1:1000) with HepG2 cells lysate 6000ug.
IHC staining of human normal colon using 10894-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10894-1-AP (CYB5R3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10894-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung cancer using 10894-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 10894-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (10894-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

-/ -

Cat No. 10894-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, MCF-7 cells, human lung tissue, human liver tissue, mouse liver tissue, human placenta tissue, HeLa cells, HepG2 cells, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human normal colon, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 22 publications below
IHC	See 5 publications below
IF	See 7 publications below

Product Information

10894-1-AP targets CYB5R3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat, pig, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CYB5R3 fusion protein Ag1339 相同性解析による交差性が予測される生物種
Full Name	cytochrome b5 reductase 3
Calculated molecular weight	33 kDa
Observed molecular weight	30-35 kDa
GenBank accession number	BC004821
Gene symbol	CYB5R3
Gene ID (NCBI)	1727
RRID	AB_2292715
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Protocols

Product Specific Protocols
WB protocol for CYB5R3 antibody 10894-1-AP	Download protocol
IHC protocol for CYB5R3 antibody 10894-1-AP	Download protocol
IF protocol for CYB5R3 antibody 10894-1-AP	Download protocol
IP protocol for CYB5R3 antibody 10894-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Sci Transl Med Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes Authors - Hitoshi Watanabe KO Validated View Article
		J Cell Biol Dia1-dependent adhesions are required by epithelial tissues to initiate invasion. Authors - Tim B Fessenden View Article
human,mouse	WB,IF	JCI Insight CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling Authors - Marta Bueno View Article
rat	IHC	Brain Struct Funct High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain. Authors - A K Samhan-Arias View Article
mouse	IHC	Blood Adv Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice. Authors - Katherine C Wood View Article
mouse	WB	Free Radic Biol Med Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3 Authors - Luz Marina Sánchez-Mendoza View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYB5R3 Polyclonal antibody

CYB5R3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 10894-1-AP

WB analysis using 10894-1-AP

WB analysis using 10894-1-AP

WB analysis of human lung using 10894-1-AP

WB analysis of MCF-7 using 10894-1-AP

WB analysis of A431 using 10894-1-AP

WB analysis of human placenta using 10894-1-AP

WB analysis of human liver using 10894-1-AP

IP experiment of HepG2 using 10894-1-AP

IHC staining of human normal colon using 10894-1-AP

IHC staining of human lung cancer using 10894-1-AP

IHC staining of human lung cancer using 10894-1-AP

IF Staining of HeLa using 10894-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes

Dia1-dependent adhesions are required by epithelial tissues to initiate invasion.

CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling

High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.

Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.

Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3