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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CYB5R3 Polyclonal antibody (CL594-10894)

    IF Staining of HeLa using CL594-10894

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CYB5R3 antibody (CL594-10894) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated CYB5R3 Polyclonal antibody (CL594-10894)

    FC experiment of HeLa using CL594-10894

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CYB5R3 (CL594-10894) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CYB5R3 Polyclonal antibody

CYB5R3 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-10894

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

NADH-cytochrome b5 reductase 3, EC:1.6.2.2, Diaphorase-1, Diaphorase 1, DIA1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10894 targets CYB5R3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYB5R3 fusion protein Ag1339 相同性解析による交差性が予測される生物種
Full Name cytochrome b5 reductase 3
Calculated molecular weight 33 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC004821
Gene Symbol CYB5R3
Gene ID (NCBI) 1727
RRIDAB_2919751
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00387
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Protocols

Product Specific Protocols
IF protocol for CL594 CYB5R3 antibody CL594-10894Download protocol
FC protocol for CL594 CYB5R3 antibody CL594-10894Download protocol
Standard Protocols
Click here to view our Standard Protocols