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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CYB5R3 Monoclonal antibody

CYB5R3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

9C4D10

Cat no : CL594-66022

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated CYB5R3 Monoclonal antibody (CL594-66022)

    IF Staining of HepG2 using CL594-66022

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66022 (CYB5R3 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CYB5R3 Monoclonal antibody (CL594-66022)

    IF Staining of HeLa using CL594-66022

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CYB5R3 antibody (CL594-66022, Clone: 9C4D10 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66022 targets CYB5R3 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYB5R3 fusion protein Ag18096 相同性解析による交差性が予測される生物種
Full Name cytochrome b5 reductase 3
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC004821
Gene symbol CYB5R3
Gene ID (NCBI) 1727
RRIDAB_2883481
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Protocols

Product Specific Protocols
IF protocol for CL594 CYB5R3 antibody CL594-66022Download protocol
Standard Protocols
Click here to view our Standard Protocols