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CYP1A1 Polyclonal antibody

CYP1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 13241-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cytochrome P450 1A1, CYPIA1, CYP1, CP11, AHRR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 647
Size/Concentration:  150 μL, 700 ug/ml
20 μL, 700 ug/ml
150 μL, 700 ug/ml

¥67,000/ 150 μL

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, mouse cerebellum tissue, mouse lung tissue, rat liver tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13241-1-AP targets CYP1A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse, rat, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP1A1 fusion protein Ag3990 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 1, subfamily A, polypeptide 1
Calculated molecular weight 512 aa, 58 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberBC023019
Gene Symbol CYP1A1
Gene ID (NCBI) 1543
RRIDAB_2877928
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04798
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP1A1 antibody 13241-1-APDownload protocol
IHC protocol for CYP1A1 antibody 13241-1-APDownload protocol
IF protocol for CYP1A1 antibody 13241-1-APDownload protocol
IP protocol for CYP1A1 antibody 13241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Environ Int

Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR)

Authors - Séverine A Degrelle
human,mouseWB

Free Radic Biol Med

5-Methoxyflavone ameliorates non-alcoholic fatty liver disease through targeting the cytochrome P450 1A1

Authors - Yurou Zhang
human,mouseWB

Phytother Res

Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity

Authors - Xiaojing Wang
mouseIF

NPJ Regen Med

Myeloid cell-associated aromatic amino acid metabolism facilitates CNS myelin regeneration

Authors - Jingwen Hu
humanWB

Environ Pollut

Regioselective hydroxylation of carbendazim by mammalian cytochrome P450: A combined experimental and computational study.

Authors - Xia Lv
humanWB,IHC

BMC Med

NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma

Authors - Yizhi Zhan
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