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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated CYP1A1 Polyclonal antibody

CYP1A1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13241

Synonyms

Cytochrome P450 1A1, CYPIA1, CYP1, CP11, AHRR

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated CYP1A1 Polyclonal an (CL647-13241)

    FC experiment of HepG2 using CL647-13241

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated CYP1A1 Polyclonal antibody (CL647-13241)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13241 targets CYP1A1 in FC (Intra) applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP1A1 fusion protein Ag3990 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 1, subfamily A, polypeptide 1
Calculated molecular weight 512 aa, 58 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberBC023019
Gene symbol CYP1A1
Gene ID (NCBI) 1543
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CYP1A1 antibody CL647-13241Download protocol
Standard Protocols
Click here to view our Standard Protocols