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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP2D6 Monoclonal antibody

CYP2D6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

8B11D12

Cat no : 60297-1-Ig

Synonyms

CPD6, CYP2D, CYP2D@, CYP2D6, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CYP2D6 Monoclonal antibody (60297-1-Ig)

    WB analysis of HepG2 using 60297-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60297-1-Ig (CYP2D6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using CYP2D6 Monoclonal antibody (60297-1-Ig)

    WB analysis of fetal human brain using 60297-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60297-1-Ig (CYP2D6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2D6 Monoclonal antibody (60297-1-Ig)

    IHC staining of human liver cancer using 60297-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60297-1-Ig (CYP2D6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2D6 Monoclonal antibody (60297-1-Ig)

    IHC staining of human liver cancer using 60297-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60297-1-Ig (CYP2D6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, fetal human brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60297-1-Ig targets CYP2D6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYP2D6 fusion protein Ag12605 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily D, polypeptide 6
Calculated molecular weight 497 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC075023
Gene symbol CYP2D6
Gene ID (NCBI) 1565
RRIDAB_2881412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for CYP2D6 antibody 60297-1-IgDownload protocol
IHC protocol for CYP2D6 antibody 60297-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols