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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CYP2D6 Monoclonal antibody (60297-1-Ig)

    WB analysis of HepG2 using 60297-1-Ig (same clone as 60297-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60297-1-Ig (CYP2D6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60297-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using CYP2D6 Monoclonal antibody (60297-1-Ig)

    WB analysis of fetal human brain using 60297-1-Ig (same clone as 60297-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60297-1-Ig (CYP2D6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60297-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2D6 Monoclonal antibody (60297-1-Ig)

    IHC staining of human liver cancer using 60297-1-Ig (same clone as 60297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60297-1-Ig (CYP2D6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60297-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2D6 Monoclonal antibody (60297-1-Ig)

    IHC staining of human liver cancer using 60297-1-Ig (same clone as 60297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60297-1-Ig (CYP2D6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60297-1-PBS in a different storage buffer formulation.

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CYP2D6 Monoclonal antibody, PBS Only

CYP2D6 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60297-1-PBS
Clone No.8B11D12

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CPD6, CYP2D, CYP2D@, CYP2D6, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60297-1-PBS targets CYP2D6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYP2D6 fusion protein Ag12605 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily D, polypeptide 6
Calculated molecular weight 497 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC075023
Gene Symbol CYP2D6
Gene ID (NCBI) 1565
RRIDAB_2881412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10635
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa.