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Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using CYP2E1-Specific Monoclonal antibody (67263-1-Ig)

    WB analysis of pig liver using 67263-1-Ig (same clone as 67263-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HaCaT cells using CYP2E1-Specific Monoclonal antibody (67263-1-Ig)

    WB analysis of HaCaT using 67263-1-Ig (same clone as 67263-1-PBS)

    HaCaT cells were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using CYP2E1-Specific Monoclonal antibody (67263-1-Ig)

    WB analysis of rat liver using 67263-1-Ig (same clone as 67263-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CYP2E1-Specific Monoclonal antibody (67263-1-Ig)

    WB analysis of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2E1-Specific Monoclonal antibody (67263-1-Ig)

    IHC staining of human liver cancer using 67263-1-Ig (same clone as 67263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP2E1-Specific Monoclonal antibody (67263-1-Ig)

    IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP2E1-Specific Monoclonal antibody (67263-1-Ig)

    IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2E1-Specific Monoclonal antibody (67263-1-Ig)

    IHC staining of human liver cancer using 67263-1-Ig (same clone as 67263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CYP2E1-Specific Monoclonal antibody (67263-1-Ig)

    IF Staining of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CYP2E1-Specific antibody (67263-1-Ig, Clone: 1F12G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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CYP2E1-Specific Monoclonal antibody, PBS Only

CYP2E1-Specific Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67263-1-PBS
Clone No.1F12G7

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CYP2E1, CYPIIE1, CYP2E, CPE1, 4-nitrophenol 2-hydroxylase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67263-1-PBS targets CYP2E1-Specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat, pig samples.

Tested Reactivity mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily E, polypeptide 1
Calculated molecular weight 57 kDa
Observed molecular weight 50-57 kDa
GenBank accession numberNM_000773
Gene Symbol CYP2E1
Gene ID (NCBI) 1571
RRIDAB_2882534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05181
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.