Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
67263-1-PBS targets CYP2E1-Specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat, pig samples.
Tested Reactivity | mouse, rat, pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | cytochrome P450, family 2, subfamily E, polypeptide 1 |
Calculated molecular weight | 57 kDa |
Observed molecular weight | 50-57 kDa |
GenBank accession number | NM_000773 |
Gene Symbol | CYP2E1 |
Gene ID (NCBI) | 1571 |
RRID | AB_2882534 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P05181 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.