CYP2E1-Specific Monoclonal antibody
CYP2E1-Specific Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
mouse, rat, pig, human
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1F12G7
Cat no : 67263-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig liver tissue, rat liver tissue, mouse liver tissue |
| Positive IHC detected in | human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
67263-1-Ig targets CYP2E1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat, pig, human samples.
| Tested Reactivity | mouse, rat, pig, human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 2, subfamily E, polypeptide 1 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 50-57 kDa |
| GenBank accession number | NM_000773 |
| Gene symbol | CYP2E1 |
| Gene ID (NCBI) | 1571 |
| RRID | AB_2882534 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP2E1-Specific antibody 67263-1-Ig | Download protocol |
| IHC protocol for CYP2E1-Specific antibody 67263-1-Ig | Download protocol |
| IF protocol for CYP2E1-Specific antibody 67263-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother The effects of scoparone on alcohol and high-fat diet-induced liver injury revealed by RNA sequencing | ||
Front Pharmacol Methyl 6-O-cinnamoyl-α-d-glucopyranoside Ameliorates Acute Liver Injury by Inhibiting Oxidative Stress Through the Activation of Nrf2 Signaling Pathway. | ||
Med Sci Monit Comparison of the Effects of Intraperitoneal Injection with Carbon Tetrachloride on Acute Liver Toxicity in Male and Female Kunming Mice | ||
Nutrients Daucosterol Alleviates Alcohol−Induced Hepatic Injury and Inflammation through P38/NF−κB/NLRP3 Inflammasome Pathway |
