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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CYP2E1-Specific Monoclonal antibody

CYP2E1-Specific Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, rat, pig, human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F12G7

Cat no : CL488-67263

Synonyms

4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 488-conjugated CYP2E1-Specific Mono (CL488-67263)

    IF Staining of mouse liver using CL488-67263

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 CYP2E1-Specific antibody (CL488-67263, Clone: 1F12G7 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 488-conjugated CYP2E1-Specific Mono (CL488-67263)

    IF Staining of mouse liver using CL488-67263

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 CYP2E1-Specific antibody (CL488-67263, Clone: 1F12G7 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67263 targets CYP2E1-Specific in IF-P applications and shows reactivity with mouse, rat, pig, human samples.

Tested Reactivity mouse, rat, pig, human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily E, polypeptide 1
Calculated molecular weight 57 kDa
Observed molecular weight 50-57 kDa
GenBank accession numberNM_000773
Gene symbol CYP2E1
Gene ID (NCBI) 1571
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CYP2E1-Specific antibody CL488-67263Download protocol
Standard Protocols
Click here to view our Standard Protocols