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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis using 67110-1-Ig (same clone as 67110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis of pig liver using 67110-1-Ig (same clone as 67110-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig gall bladder tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis of pig gall bladder using 67110-1-Ig (same clone as 67110-1-PBS)

    pig gall bladder tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis of mouse lung using 67110-1-Ig (same clone as 67110-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IHC staining of human liver cancer using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IHC staining of human liver cancer using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IF Staining of rat liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IF Staining of HepG2 using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50718-4

    Cytometric bead array standard curve of MP50718-4

    Cytometric bead array standard curve of MP50718-4, CYP3A4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67110-6-PBS. Detection antibody: 67110-1-PBS. Standard:Ag13074. Range: 0.781-100 ng/mL.

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CYP3A4 Monoclonal antibody, PBS Only (Detector)

CYP3A4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67110-1-PBS
Clone No.2F11C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

CYP3A3, CYP3A, CP34, CP33, Cholesterol 25-hydroxylase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67110-1-PBS targets CYP3A4 as part of a matched antibody pair:

MP50718-4: 67110-6-PBS capture and 67110-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP3A4 fusion protein Ag13074 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 3, subfamily A, polypeptide 4
Calculated molecular weight 503 aa, 57 kDa
Observed molecular weight 51-57 kDa
GenBank accession numberBC101631
Gene Symbol CYP3A4
Gene ID (NCBI) 1576
RRIDAB_2882414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08684
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.