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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP3A4 Monoclonal antibody

CYP3A4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2F11C4

Cat no : 67110-1-Ig

Synonyms

CYP3A3, CYP3A, CP34, CP33, Cholesterol 25-hydroxylase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis of mouse liver using 67110-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis using 67110-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis of pig liver using 67110-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig gall bladder tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis of pig gall bladder using 67110-1-Ig

    pig gall bladder tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    WB analysis of mouse lung using 67110-1-Ig

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IHC staining of mouse liver using 67110-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IHC staining of mouse liver using 67110-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IHC staining of human liver cancer using 67110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IHC staining of human liver cancer using 67110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IF Staining of mouse liver using 67110-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IF Staining of mouse liver using 67110-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IF Staining of rat liver using 67110-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP3A4 Monoclonal antibody (67110-1-Ig)

    IF Staining of HepG2 using 67110-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inmouse liver tissue, pig liver tissue, pig gall bladder tissue, mouse lung tissue, rat liver tissue
Positive IHC detected inmouse liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue, rat liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 3 publications below

Product Information

67110-1-Ig targets CYP3A4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP3A4 fusion protein Ag13074 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 3, subfamily A, polypeptide 4
Calculated molecular weight 503 aa, 57 kDa
Observed molecular weight 51-57 kDa
GenBank accession numberBC101631
Gene symbol CYP3A4
Gene ID (NCBI) 1576
RRIDAB_2882414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP3A4 antibody 67110-1-IgDownload protocol
IHC protocol for CYP3A4 antibody 67110-1-IgDownload protocol
IF protocol for CYP3A4 antibody 67110-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Biomed Rep

Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells.

Authors - Rong Li
View Article
humanIF

Methods Mol Biol

Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells.

Authors - Rong Li
View Article
humanIF

Acta Biomater

Hepar-on-a-sensor-platform with hybridization chain reaction amplification strategy to intuitively monitor the hepatoxicity of natural compounds

Authors - Wenhui Qian
View Article