CoraLite® Plus 488-conjugated CYP3A4 Monoclonal antibody

CYP3A4 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F11C4

Cat no : CL488-67110

Synonyms

CYP3A3, CYP3A, CP34, CP33, Cholesterol 25-hydroxylase


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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL488-67110 targets CYP3A4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP3A4 fusion protein Ag13074 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 3, subfamily A, polypeptide 4
Calculated molecular weight 503 aa, 57 kDa
Observed molecular weight 51-57 kDa
GenBank accession numberBC101631
Gene symbol CYP3A4
Gene ID (NCBI) 1576
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The murine homolog of human CYP3A4 is CYP3A11 (PMID: 19297428). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CYP3A4 antibody CL488-67110Download protocol
FC protocol for CL Plus 488 CYP3A4 antibody CL488-67110Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

ACS Appl Bio Mater

3D Bioprinting of Sugar Beet Pectin through Horseradish Peroxidase-Catalyzed Cross-Linking

Authors - Wildan Mubarok