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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP51A1 Recombinant antibody (84705-4-RR)

    WB analysis using 84705-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84705-4-RR (CYP51A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84705-4-RR

    Affinity and Kinetic Characterization of 84705-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84705-4-RR against Human CYP51A1 were performed. The affinity constant is below 1 pM.

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CYP51A1 Recombinant antibody

CYP51A1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84705-4-RR
Clone No.242166G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Cytochrome P450 14DM, CYPLI, CYPL1, CYP51, CP51

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HuH-7 cells, HEK-293 cells, RAW 264.7 cells, mouse testis tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84705-4-RR targets CYP51A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CYP51A1 fusion protein Ag4235 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 51, subfamily A, polypeptide 1
Calculated molecular weight 509 aa, 57 kDa
Observed molecular weight56 kDa
GenBank accession numberBC032322
Gene Symbol CYP51A1
Gene ID (NCBI) 1595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16850
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) belongs to the evolutionarily conserved family of cytochrome P450 and catalyzes one of the key steps in cholesterol biosynthesis. CYP51A1 protein is targeted for degradation when exposed to nitric oxide generated under inflammatory conditions by NOS2 or released from NO donor compounds (PMID: 28830911). CYP51A1 is a prognostic marker in certain cancers, including Cervical squamous cell carcinoma, Head and neck squamous cell carcinoma, Kidney chromophobe, and Kidney renal clear cell carcinoma (PMID: 24711211).

Protocols

Product Specific Protocols
WB protocol for CYP51A1 antibody 84705-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols