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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP7A1 Polyclonal antibody (18054-1-AP)

    WB analysis using 18054-1-AP (same clone as 18054-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18054-1-AP (CYP7A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP7A1 Polyclonal antibody (18054-1-AP)

    IHC staining of human liver cancer using 18054-1-AP (same clone as 18054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP7A1 Polyclonal antibody (18054-1-AP)

    IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP7A1 Polyclonal antibody (18054-1-AP)

    IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP7A1 Polyclonal antibody (18054-1-AP)

    IHC staining of human liver cancer using 18054-1-AP (same clone as 18054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP7A1 Polyclonal antibody (18054-1-AP)

    IF Staining of HepG2 using 18054-1-AP (same clone as 18054-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7A1 antibody (18054-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

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CYP7A1 Polyclonal antibody, PBS Only

CYP7A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18054-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Cytochrome P450 7A1, CYPVII, CYP7, CP7A, Cholesterol 7-alpha-monooxygenase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18054-1-PBS targets CYP7A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP7A1 fusion protein Ag12572 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 7, subfamily A, polypeptide 1
Calculated molecular weight 504 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC101777
Gene Symbol CYP7A1
Gene ID (NCBI) 1581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22680
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1), is a cholesterol 7alpha-hydroxylase, catalyzing the first and rate-limiting step in the neutral or classic pathway for bile acid biosynthesis. CYP7A1 is a postulated gene modifier of colorectal cancer risk and target for the therapeutic bile acid, ursodeoxycholic acid (UDCA). The CYP7A1 enzyme controls the rate-limiting step of the catabolism of cholesterol into bile acids in the liver.