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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CYR61/CCN1 Monoclonal antibody

CYR61/CCN1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E11F11

Cat no : CL488-67656

Synonyms

CYR61, Cysteine-rich angiogenic inducer 61, Cellular communication network factor 1, CCN1, CCN family member 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated CYR61 Monoclonal ant (CL488-67656)

    IF Staining of MCF-7 using CL488-67656

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 CYR61 antibody (CL488-67656, Clone: 2E11F11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CYR61 Monoclonal ant (CL488-67656)

    IF Staining of HeLa using CL488-67656

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CYR61 antibody (CL488-67656, Clone: 2E11F11 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67656 targets CYR61/CCN1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYR61/CCN1 fusion protein Ag25129 相同性解析による交差性が予測される生物種
Full Name cysteine-rich, angiogenic inducer, 61
Calculated molecular weight 42 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC001271
Gene symbol CYR61
Gene ID (NCBI) 3491
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CYR61/CCN1 antibody CL488-67656Download protocol
Standard Protocols
Click here to view our Standard Protocols