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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calbindin-D28k Monoclonal antibody, PBS Only

Calbindin-D28k Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F8B9

Cat no : 66394-1-PBS

Synonyms

CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, Calbindin-D28k, D 28K

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    WB analysis using 66394-1-Ig (same clone as 66394-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    WB analysis of PC-12 using 66394-1-Ig (same clone as 66394-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    WB analysis of fetal human brain using 66394-1-Ig (same clone as 66394-1-PBS)

    Human brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of rat cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of rat cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of human brain using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of human brain using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of mouse kidney using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of mouse kidney using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of rat kidney using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of rat kidney using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of human kidney using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of human kidney using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of human small intestine using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IHC staining of human small intestine using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IF Staining of mouse cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IF Staining of mouse cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IF Staining of human kidney using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:300 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Podocalyxin antibody(18150-1-AP, green). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IF Staining of mouse cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IF Staining of mouse cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Calbindin-D28k Monoclonal antibody (66394-1-Ig)

    IF Staining of PC-12 using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66394-1-PBS targets Calbindin-D28k in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calbindin-D28k fusion protein Ag5861 相同性解析による交差性が予測される生物種
Full Name calbindin 1, 28kDa
Calculated molecular weight 30 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC006478
Gene symbol CALB1
Gene ID (NCBI) 793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.