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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Calpastatin Monoclonal antibody

Calpastatin Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G12A2

Cat no : CL594-67107

Synonyms

BS 17, Calpain inhibitor, calpastatin, CAST, Sperm BS 17 component

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Calpastatin Monoclonal ant (CL594-67107)

    IF Staining of HeLa using CL594-67107

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Calpastatin antibody (CL594-67107, Clone: 1G12A2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Calpastatin Monoclonal ant (CL594-67107)

    FC experiment of HeLa using CL594-67107

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Calpastatin (CL594-67107, Clone:1G12A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67107 targets Calpastatin in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calpastatin fusion protein Ag28669 相同性解析による交差性が予測される生物種
Full Name calpastatin
Calculated molecular weight 667 aa, 72 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC013579
Gene symbol Calpastatin
Gene ID (NCBI) 831
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 Calpastatin antibody CL594-67107Download protocol
FC protocol for CL594 Calpastatin antibody CL594-67107Download protocol
Standard Protocols
Click here to view our Standard Protocols