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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calponin Monoclonal antibody

Calponin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11E5

Cat no : 66540-1-Ig

Synonyms

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calponin Monoclonal antibody (66540-1-Ig)

    WB analysis using 66540-1-Ig

    Various tissue were subjected to SDS PAGE followed by western blot with 66540-1-Ig (Calponin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human myofibroblastoma tissue using Calponin Monoclonal antibody (66540-1-Ig)

    IHC staining of human myofibroblastoma using 66540-1-Ig

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human myofibroblastoma tissue using Calponin Monoclonal antibody (66540-1-Ig)

    IHC staining of human myofibroblastoma using 66540-1-Ig

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman heart tissue, human vein tissue, human colon tissue, human ileum tissue, human bladder tissue, human kidney tissue
Positive IHC detected inhuman myofibroblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1500-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66540-1-Ig targets Calponin in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Calponin fusion protein Ag21384 相同性解析による交差性が予測される生物種
Full Name calponin 1, basic, smooth muscle
Calculated molecular weight 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC022015
Gene symbol Calponin
Gene ID (NCBI) 1264
RRIDAB_2881902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.

Protocols

Product Specific Protocols
WB protocol for Calponin antibody 66540-1-IgDownload protocol
IHC protocol for Calponin antibody 66540-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols