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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calponin Monoclonal antibody, PBS Only (Detector)

Calponin Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11E5

Cat no : 66540-1-PBS

Synonyms

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calponin Monoclonal antibody (66540-1-Ig)

    WB analysis using 66540-1-Ig (same clone as 66540-1-PBS)

    Various tissue were subjected to SDS PAGE followed by western blot with 66540-1-Ig (Calponin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50017-1

    Sandwich ELISA standard curve of MP50017-1

    Sandwich ELISA standard curve ofMP50017-1, Calponin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66540-2-PBS. Detection antibody: HRP-conjugated 66540-1-PBS. Standard: Ag21384. Range: 0.313-20 ng/mL.

  • Immunohistochemistry (IHC) staining of human myofibroblastoma tissue using Calponin Monoclonal antibody (66540-1-Ig)

    IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human myofibroblastoma tissue using Calponin Monoclonal antibody (66540-1-Ig)

    IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66540-1-PBS targets Calponin as part of a matched antibody pair:

MP50017-1: 66540-2-PBS capture and 66540-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Calponin fusion protein Ag21384 相同性解析による交差性が予測される生物種
Full Name calponin 1, basic, smooth muscle
Calculated molecular weight 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC022015
Gene symbol Calponin
Gene ID (NCBI) 1264
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.