Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 12278-1-AP (same clone as 12278-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12278-1-AP (same clone as 12278-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
WB analysis of human colon using 12278-1-AP (same clone as 12278-1-PBS)
human colon tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
WB analysis of human ileum using 12278-1-AP (same clone as 12278-1-PBS)
human ileum tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
WB analysis of fetal human brain using 12278-1-AP (same clone as 12278-1-PBS)
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)
IP result of anti-Calretinin (IP:12278-1-AP, 4ug; Detection:12278-1-AP 1:10000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human cerebellum using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human cerebellum using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

Calretinin Polyclonal antibody, PBS Only

Calretinin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12278-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12278-1-PBS targets Calretinin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Calretinin fusion protein Ag2924 相同性解析による交差性が予測される生物種
Full Name	calbindin 2
Calculated molecular weight	29 kDa
Observed molecular weight	29 kDa
GenBank accession number	BC015484
Gene Symbol	Calretinin
Gene ID (NCBI)	794
RRID	AB_2228338
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22676
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12278-1-AP (same clone as 12278-1-PBS)

WB analysis of human brain using 12278-1-AP (same clone as 12278-1-PBS)

WB analysis of human colon using 12278-1-AP (same clone as 12278-1-PBS)

WB analysis of human ileum using 12278-1-AP (same clone as 12278-1-PBS)

WB analysis of fetal human brain using 12278-1-AP (same clone as 12278-1-PBS)

IP experiment of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)

IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)