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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calretinin Recombinant antibody (82811-1-RR)

    WB analysis using 82811-1-RR (same clone as 82811-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82811-1-RR (Calretinin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Calretinin Recombinant antibody (82811-1-RR)

    IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Calretinin Recombinant antibody (82811-1-RR)

    IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of adult mouse cerebellum tissue using Calretinin Recombinant antibody (82811-1-RR)

    IF Staining of adult mouse cerebellum tissue using 82811-1-RR (same clone as 82811-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Calretinin antibody (82811-1-RR) at dilution of 1:500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Calretinin Recombinant antibody (82811-1-RR)

    IF Staining of SH-SY5Y using 82811-1-RR (same clone as 82811-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Calretinin antibody (82811-1-RR, Clone: 2F20 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Calretinin Recombinant antibody (82811-1-RR)

    FC experiment of SH-SY5Y using 82811-1-RR (same clone as 82811-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Calretinin Recombinant antibody (82811-1-RR, Clone:2F20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

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Calretinin Recombinant antibody, PBS Only

Calretinin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82811-1-PBS
Clone No.2F20

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CALB2, calbindin 2, CAL2, CAB29, 2F20

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82811-1-PBS targets Calretinin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Calretinin fusion protein Ag2924 相同性解析による交差性が予測される生物種
Full Name calbindin 2
Calculated molecular weight 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC015484
Gene Symbol Calretinin
Gene ID (NCBI) 794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22676
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.