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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calretinin Recombinant antibody

Calretinin Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F20

Cat no : 82811-1-RR

Synonyms

CALB2, calbindin 2, CAL2, CAB29, 2F20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calretinin Recombinant antibody (82811-1-RR)

    WB analysis using 82811-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82811-1-RR (Calretinin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Calretinin Recombinant antibody (82811-1-RR)

    IHC staining of mouse brain using 82811-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Calretinin Recombinant antibody (82811-1-RR)

    IHC staining of mouse brain using 82811-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of adult mouse cerebellum tissue using Calretinin Recombinant antibody (82811-1-RR)

    IF Staining of adult mouse cerebellum tissue using 82811-1-RR

    Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Calretinin antibody (82811-1-RR) at dilution of 1:500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University)

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Calretinin Recombinant antibody (82811-1-RR)

    IF Staining of SH-SY5Y using 82811-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Calretinin antibody (82811-1-RR, Clone: 2F20 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Calretinin Recombinant antibody (82811-1-RR)

    FC experiment of SH-SY5Y using 82811-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Calretinin Recombinant antibody (82811-1-RR, Clone:2F20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inSH-SY5Y cells, U2OS cells, fetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5100
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82811-1-RR targets Calretinin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Calretinin fusion protein Ag2924 相同性解析による交差性が予測される生物種
Full Name calbindin 2
Calculated molecular weight 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC015484
Gene symbol Calretinin
Gene ID (NCBI) 794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Protocols

Product Specific Protocols
WB protocol for Calretinin antibody 82811-1-RRDownload protocol
IHC protocol for Calretinin antibody 82811-1-RRDownload protocol
IF protocol for Calretinin antibody 82811-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols