Calretinin Recombinant antibody
Calretinin Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
2F20
Cat no : 82811-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SH-SY5Y cells, U2OS cells, fetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells |
Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5100 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82811-1-RR targets Calretinin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Calretinin fusion protein Ag2924 相同性解析による交差性が予測される生物種 |
Full Name | calbindin 2 |
Calculated molecular weight | 29 kDa |
Observed molecular weight | 29 kDa |
GenBank accession number | BC015484 |
Gene symbol | Calretinin |
Gene ID (NCBI) | 794 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.
Protocols
Product Specific Protocols | |
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WB protocol for Calretinin antibody 82811-1-RR | Download protocol |
IHC protocol for Calretinin antibody 82811-1-RR | Download protocol |
IF protocol for Calretinin antibody 82811-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |