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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calumenin Monoclonal antibody

Calumenin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D8B8

Cat no : 67585-1-Ig

Synonyms

CALU, calumenin, Crocalbin, FLJ90608, IEF SSP 9302

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calumenin Monoclonal antibody (67585-1-Ig)

    WB analysis using 67585-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67585-1-Ig (CALU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human colon cancer using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human colon cancer using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human cervical cancer using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human cervical cancer using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human kidney using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human kidney using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of mouse placenta using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of mouse placenta using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Calumenin Monoclonal antibody (67585-1-Ig)

    IF Staining of HeLa using 67585-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (67585-1-Ig, Clone: 2D8B8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, Jurkat cells, RAW264.7 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human kidney tissue, mouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67585-1-Ig targets Calumenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calumenin fusion protein Ag12073 相同性解析による交差性が予測される生物種
Full Name calumenin
Calculated molecular weight 315 aa, 37 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC013383
Gene symbol Calumenin
Gene ID (NCBI) 813
RRIDAB_2882794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Calumenin antibody 67585-1-IgDownload protocol
IHC protocol for Calumenin antibody 67585-1-IgDownload protocol
IF protocol for Calumenin antibody 67585-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols