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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calumenin Monoclonal antibody, PBS Only (Detector)

Calumenin Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8B8

Cat no : 67585-1-PBS

Synonyms

CALU, IEF SSP 9302, Crocalbin, 2D8B8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calumenin Monoclonal antibody (67585-1-Ig)

    WB analysis using 67585-1-Ig (same clone as 67585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67585-1-Ig (CALU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human colon cancer using 67585-1-Ig (same clone as 67585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human colon cancer using 67585-1-Ig (same clone as 67585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human cervical cancer using 67585-1-Ig (same clone as 67585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human cervical cancer using 67585-1-Ig (same clone as 67585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human kidney using 67585-1-Ig (same clone as 67585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of human kidney using 67585-1-Ig (same clone as 67585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of mouse placenta using 67585-1-Ig (same clone as 67585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using Calumenin Monoclonal antibody (67585-1-Ig)

    IHC staining of mouse placenta using 67585-1-Ig (same clone as 67585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Calumenin Monoclonal antibody (67585-1-Ig)

    IF Staining of HeLa using 67585-1-Ig (same clone as 67585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (67585-1-Ig, Clone: 2D8B8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50698-2

    Cytometric bead array standard curve of MP50698-2

    Cytometric bead array standard curve of MP50698-2, CALU Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67585-2-PBS. Detection antibody: 67585-1-PBS. Standard:Ag12073. Range: 0.195-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67585-1-PBS targets Calumenin as part of a matched antibody pair:

MP50698-2: 67585-2-PBS capture and 67585-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calumenin fusion protein Ag12073 相同性解析による交差性が予測される生物種
Full Name calumenin
Calculated molecular weight 315 aa, 37 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC013383
Gene symbol Calumenin
Gene ID (NCBI) 813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.