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CoraLite® Plus 488-conjugated Caspase 3/p17/p19 Monoclonal antibody
Caspase 3/p17/p19 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2G4B2
Cat no : CL488-66470
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse brain tissue, human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66470 targets Caspase 3/p17/p19 in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Caspase 3/p17/p19 fusion protein Ag25029 相同性解析による交差性が予測される生物種 |
| Full Name | caspase 3, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 277 aa, 32 kDa |
| Observed molecular weight | 32-35 kDa, 19 kDa, 17 kDa |
| GenBank accession number | BC016926 |
| Gene symbol | Caspase 3 |
| Gene ID (NCBI) | 836 |
| RRID | AB_2919331 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Caspase 3/p17/p19 antibody CL488-66470 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
