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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Caspase 3/p17/p19 Polyclonal antibody

Caspase 3/p17/p19 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-19677

Synonyms

CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite®594-conjugated Caspase 3/p17/p19 Polyclon (CL594-19677)

    IF Staining of NIH/3T3 using CL594-19677

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®594 Caspase 3/p17/p19 antibody (CL594-19677) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite®594-conjugated Caspase 3/p17/p19 Polyclon (CL594-19677)

    IF Staining of NIH/3T3 using CL594-19677

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite®594 Caspase 3/p17/p19 antibody (CL594-19677) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated Caspase 3/p17/p19 Polyclon (CL594-19677)

    FC experiment of HepG2 using CL594-19677

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Caspase 3/p17/p19 (CL594-19677) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-19677 targets Caspase 3/p17/p19 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight 32 kDa
Observed molecular weight 32-35 kDa, 17 kDa, 19 kDa
GenBank accession numberNM_004346
Gene symbol Caspase 3
Gene ID (NCBI) 836
RRIDAB_2919853
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form a heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19, and p32 of Caspase 3.

Protocols

Product Specific Protocols
IF protocol for CL594 Caspase 3/p17/p19 antibody CL594-19677Download protocol
FC protocol for CL594 Caspase 3/p17/p19 antibody CL594-19677Download protocol
Standard Protocols
Click here to view our Standard Protocols