Caspase 4 Monoclonal antibody
Caspase 4 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1D1E12
Cat no : 67398-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SGC-7901 cells, A549 cells, HeLa cells, HepG2 cells, HL-60 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
67398-1-Ig targets Caspase 4 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Caspase 4 fusion protein Ag29488 相同性解析による交差性が予測される生物種 |
| Full Name | caspase 4, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 377 aa, 43 kDa |
| Observed molecular weight | 43-48 kDa |
| GenBank accession number | BC017839 |
| Gene symbol | Caspase 4 |
| Gene ID (NCBI) | 837 |
| RRID | AB_2882641 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 4 antibody 67398-1-Ig | Download protocol |
| IHC protocol for Caspase 4 antibody 67398-1-Ig | Download protocol |
| IF protocol for Caspase 4 antibody 67398-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Cell Pyroptosis: a novel signature to predict prognosis and immunotherapy response in gliomas | ||
Life Sci Alliance Caspase-4 dimerisation and D289 auto-processing elicit an interleukin-1β-converting enzyme |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Dave (Verified Customer) (06-13-2022) | This is a great antibody. Work very efficiently if you use it as the first antibody (worse less well if your membrane has already been deactivated with Peroxide). This product also detect efficiently cleaved Caspase-4 (which can be very challenging with other antibodies)
|
FH Aldona (Verified Customer) (10-29-2021) | I have no comments
|
