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Validation Data Gallery

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  • Western Blot (WB) analysis of SGC-7901 cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of SGC-7901 using 67398-1-Ig

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of HL-60 using 67398-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of A549 using 67398-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of HepG2 using 67398-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of HeLa using 67398-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IHC staining of human liver cancer using 67398-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IHC staining of human liver cancer using 67398-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IF Staining of human liver cancer using 67398-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IF Staining of human liver cancer using 67398-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IF Staining of HeLa using 67398-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Caspase 4 Monoclonal antibody

Caspase 4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67398-1-Ig
Clone No.1D1E12
Publications(4)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CASP4, Caspase4, Caspase 4/p20/p10, CASP-4, CASP 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSGC-7901 cells, A549 cells, HeLa cells, HepG2 cells, HL-60 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67398-1-Ig targets Caspase 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caspase 4 fusion protein Ag29488 相同性解析による交差性が予測される生物種
Full Name caspase 4, apoptosis-related cysteine peptidase
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 43-48 kDa
GenBank accession numberBC017839
Gene Symbol Caspase 4
Gene ID (NCBI) 837
RRIDAB_2882641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49662
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Protocols

Product Specific Protocols
WB protocol for Caspase 4 antibody 67398-1-IgDownload protocol
IHC protocol for Caspase 4 antibody 67398-1-IgDownload protocol
IF protocol for Caspase 4 antibody 67398-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hum Cell

Pyroptosis: a novel signature to predict prognosis and immunotherapy response in gliomas

Authors - Guiying He
View Article
humanWB

Life Sci Alliance

Caspase-4 dimerisation and D289 auto-processing elicit an interleukin-1β-converting enzyme

Authors - Amy H Chan
View Article
humanWB

ACS Chem Neurosci

Hypoxanthine Produces Rapid Antidepressant Effects by Suppressing Inflammation in Serum and Hippocampus

Authors - Lei Wu
View Article
humanWB

Mol Carcinog

Pyroptosis-related gene GSDMC indicates poor prognosis and promotes tumor progression by activating the AKT/mTOR pathway in lung squamous cell carcinoma

Authors - Yi Zhang
View Article