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Validation Data Gallery

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  • Western Blot (WB) analysis of SGC-7901 cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of SGC-7901 using 67398-1-Ig (same clone as 67398-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of HL-60 using 67398-1-Ig (same clone as 67398-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of A549 using 67398-1-Ig (same clone as 67398-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of HepG2 using 67398-1-Ig (same clone as 67398-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    WB analysis of HeLa using 67398-1-Ig (same clone as 67398-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IF Staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IF Staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Caspase 4 Monoclonal antibody (67398-1-Ig)

    IF Staining of HeLa using 67398-1-Ig (same clone as 67398-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

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Caspase 4 Monoclonal antibody, PBS Only

Caspase 4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67398-1-PBS
Clone No.1D1E12

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CASP4, Caspase4, Caspase 4/p20/p10, CASP-4, CASP 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67398-1-PBS targets Caspase 4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caspase 4 fusion protein Ag29488 相同性解析による交差性が予測される生物種
Full Name caspase 4, apoptosis-related cysteine peptidase
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 43-48 kDa
GenBank accession numberBC017839
Gene Symbol Caspase 4
Gene ID (NCBI) 837
RRIDAB_2882641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49662
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.