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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Caspase 8/p43/p18 Monoclon (CL594-66093)

    IF Staining of HeLa using CL594-66093

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Caspase 8/p43/p18 antibody (CL594-66093, Clone: 2B9H8 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Caspase 8/p43/p18 Monoclon (CL594-66093)

    IF Staining of HeLa using CL594-66093

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66093 (Caspase 8/p43/p18 antibody) at dilution of 1:100.

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Caspase 8/p43/p18 Monoclonal antibody

Caspase 8/p43/p18 Monoclonal Antibody for IF/ICC
Cat No. CL594-66093
Clone No.2B9H8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Synonyms

CASP8, Caspase 8, CASP-8, CAP4, Apoptotic protease Mch-5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66093 targets Caspase 8/p43/p18 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Caspase 8/p43/p18 fusion protein Ag20524 相同性解析による交差性が予測される生物種
Full Name caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight 538 aa, 62 kDa
Observed molecular weight 53-57 kDa, 32-45 kDa, 18 kDa
GenBank accession numberBC028223
Gene Symbol Caspase 8
Gene ID (NCBI) 841
RRIDAB_2883508
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14790
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.

Protocols

Product Specific Protocols
IF protocol for CL594 Caspase 8/p43/p18 antibody CL594-66093Download protocol
Standard Protocols
Click here to view our Standard Protocols