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  • Featured Product
  • KD/KO Validated

Cathepsin D Polyclonal antibody, PBS Only

Cathepsin D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21327-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cathepsin D Polyclonal antibody (21327-1-AP)

    WB analysis using 21327-1-AP (same clone as 21327-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cathepsin D Polyclonal antibody (21327-1-AP)

    WB analysis using 21327-1-AP (same clone as 21327-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Cathepsin D Polyclonal antibody (21327-1-AP)

    IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Cathepsin D Polyclonal antibody (21327-1-AP)

    IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Cathepsin D Polyclonal antibody (21327-1-AP)

    IF Staining of MCF-7 using 21327-1-AP (same clone as 21327-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21327-1-PBS targets Cathepsin D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin D fusion protein Ag15254 相同性解析による交差性が予測される生物種
Full Name cathepsin D
Calculated molecular weight 412 aa, 45 kDa
Observed molecular weight 32 kDa, 48 kDa, 52 kDa
GenBank accession numberBC016320
Gene symbol Cathepsin D
Gene ID (NCBI) 1509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).