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  • KD/KO Validated

Cathepsin D Monoclonal antibody

Cathepsin D Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F6F7

Cat no : 66534-1-Ig

Synonyms

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain


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Tested Applications

Positive WB detected inSaos-2 cells, MCF-7 cells, T-47D cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66534-1-Ig targets Cathepsin D in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cathepsin D fusion protein Ag15254 相同性解析による交差性が予測される生物種
Full Name cathepsin D
Calculated molecular weight 412 aa, 45 kDa
Observed molecular weight 32 kDa, 48 kDa, 52 kDa
GenBank accession numberBC016320
Gene symbol Cathepsin D
Gene ID (NCBI) 1509
RRIDAB_2881897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTSD (Cathepsin D) is also named as CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)

Protocols

Product Specific Protocols
WB protocol for Cathepsin D antibody 66534-1-IgDownload protocol
IHC protocol for Cathepsin D antibody 66534-1-IgDownload protocol
IF protocol for Cathepsin D antibody 66534-1-IgDownload protocol
FC protocol for Cathepsin D antibody 66534-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Gastroenterology

Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors

Authors - Mingjun Sun
ratWB,IF

Life (Basel)

Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons.

Authors - Zhiyuan Wu
WB

Cell Rep

Intracellular complement C5a/C5aR1 stabilizes β-catenin to promote colorectal tumorigenesis.

Authors - Peipei Ding
  • KD Validated
humanIF

FEBS Lett

Senile plaques in Alzheimer's disease arise from Aβ- and Cathepsin D-enriched mixtures leaking out during intravascular haemolysis and microaneurysm rupture

Authors - Hualin Fu
human,ratWB

Ecotoxicol Environ Saf

The inhibition of TRPML1/TFEB leads to lysosomal biogenesis disorder, contributes to developmental fluoride neurotoxicity

Authors - Jingjing Zhang
ratWB

Ecotoxicol Environ Saf

TFE3-mediated impairment of lysosomal biogenesis and defective autophagy contribute to fluoride-induced hepatotoxicity

Authors - Zeyu Hu