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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cathepsin D Monoclonal antibody (66534-1-Ig)

    WB analysis using 66534-1-Ig (same clone as 66534-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Cathepsin D Monoclonal antibody (66534-1-Ig)

    WB analysis of MCF-7 using 66534-1-Ig (same clone as 66534-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cathepsin D Monoclonal antibody (66534-1-Ig)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cathepsin D Monoclonal antibody (66534-1-Ig)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cathepsin D Monoclonal antibody (66534-1-Ig)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cathepsin D Monoclonal antibody (66534-1-Ig)

    IHC staining of human breast cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cathepsin D Monoclonal antibody (66534-1-Ig)

    IHC staining of human breast cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cathepsin D Monoclonal antibody (66534-1-Ig)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cathepsin D Monoclonal antibody (66534-1-Ig)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cathepsin D Monoclonal antibody (66534-1-Ig)

    IF Staining of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cathepsin D antibody (66534-1-Ig, Clone: 2F6F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Cathepsin D Monoclonal antibody (66534-1-Ig)

    FC experiment of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cathepsin D (66534-1-Ig, Clone:2F6F7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Cathepsin D Monoclonal antibody, PBS Only

Cathepsin D Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66534-1-PBS
Clone No.2F6F7

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66534-1-PBS targets Cathepsin D in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cathepsin D fusion protein Ag15254 相同性解析による交差性が予測される生物種
Full Name cathepsin D
Calculated molecular weight 412 aa, 45 kDa
Observed molecular weight 32 kDa, 48 kDa, 52 kDa
GenBank accession numberBC016320
Gene Symbol Cathepsin D
Gene ID (NCBI) 1509
RRIDAB_2881897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07339
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).