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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    WB result of Cathepsin L antibody (66914-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCI-H1299 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of NCI-H1299 using 66914-1-Ig (same clone as 66914-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of HUVEC using 66914-1-Ig (same clone as 66914-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of HepG2 using 66914-1-Ig (same clone as 66914-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of HeLa using 66914-1-Ig (same clone as 66914-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Cathepsin L Monoclonal antibody (66914-1-Ig)

    IHC staining of human prostate cancer using 66914-1-Ig (same clone as 66914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66914-1-Ig (Cathepsin L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    IF Staining of HEK-293 using 66914-1-Ig (same clone as 66914-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Cathepsin L antibody (66914-1-Ig, Clone: 1A12G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    IF Staining of HEK-293 using 66914-1-Ig (same clone as 66914-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Cathepsin L antibody (66914-1-Ig, Clone: 1A12G8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51216-1

    Cytometric bead array standard curve of MP51216-1

    Cytometric bead array standard curve of MP51216-1, Cathepsin L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66914-2-PBS. Detection antibody: 66914-1-PBS. Standard:Ag27951. Range: 0.781-50 ng/mL.

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  • Featured Product
  • KD/KO Validated

Cathepsin L Monoclonal antibody, PBS Only (Detector)

Cathepsin L Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66914-1-PBS
Clone No.1A12G8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

CTSL1, 1A12G8, Cathepsin L heavy chain, Cathepsin L light chain, cathepsin L1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66914-1-PBS targets Cathepsin L as part of a matched antibody pair:

MP51216-1: 66914-2-PBS capture and 66914-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cathepsin L fusion protein Ag27951 相同性解析による交差性が予測される生物種
Full Name cathepsin L1
Calculated molecular weight 38 kDa
Observed molecular weight43 kDa
GenBank accession numberBC012612
Gene Symbol Cathepsin L
Gene ID (NCBI) 1514
RRIDAB_2882241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07711
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)