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  • KD/KO Validated

Cathepsin L Monoclonal antibody

Cathepsin L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66914-1-Ig
Clone No.1A12G8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

CTSL1, CATL, cathepsinL1, cathepsin L1, Cathepsin L light chain

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, NCI-H1299 cells, HUVEC cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66914-1-Ig targets Cathepsin L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cathepsin L fusion protein Ag27951 相同性解析による交差性が予測される生物種
Full Name cathepsin L1
Calculated molecular weight 38 kDa
Observed molecular weight43 kDa
GenBank accession numberBC012612
Gene Symbol Cathepsin L
Gene ID (NCBI) 1514
RRIDAB_2882241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07711
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Protocols

Product Specific Protocols
WB protocol for Cathepsin L antibody 66914-1-IgDownload protocol
IHC protocol for Cathepsin L antibody 66914-1-IgDownload protocol
IF protocol for Cathepsin L antibody 66914-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer

Authors - Cheng-Ke Xie
mouseWB

Clin Sci (Lond)

Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning

Authors - Limin Shi
ratIHC

Tissue Cell

High expression of SERPINE1 and CTSL in keratinocytes in pressure injury caused by ischemia-reperfusion injury

Authors - Lexuan Bi
humanWB

Cancer Sci

Methyltransferase-like 3 promotes cervical cancer metastasis by enhancing cathepsin L mRNA stability in an N6-methyladenosine-dependent manner

Authors - Pingping Liu
  • KD Validated
human,mouseWB

Chem Biodivers

Design, Synthesis and Biological Evaluation of Naphthalimide-Polyamine Conjugate as a Potential Anti-Colorectal Cancer Agent

Authors - Fujun Dai
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