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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Cathepsin L Monoclonal ant (CL594-66914)

    IF Staining of HeLa using CL594-66914

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Cathepsin L antibody (CL594-66914, Clone: 1A12G8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A549 cells using CoraLite®594-conjugated Cathepsin L Monoclonal ant (CL594-66914)

    FC experiment of A549 using CL594-66914

    1X10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Cathepsin L (CL594-66914, Clone:1A12G8) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO Validated

CoraLite®594-conjugated Cathepsin L Monoclonal antibody

Cathepsin L Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66914
Clone No.1A12G8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

CTSL1, CATL, cathepsinL1, cathepsin L1, Cathepsin L light chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66914 targets Cathepsin L in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cathepsin L fusion protein Ag27951 相同性解析による交差性が予測される生物種
Full Name cathepsin L1
Calculated molecular weight 38 kDa
Observed molecular weight43 kDa
GenBank accession numberBC012612
Gene Symbol Cathepsin L
Gene ID (NCBI) 1514
RRIDAB_3084735
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07711
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Protocols

Product Specific Protocols
IF protocol for CL594 Cathepsin L antibody CL594-66914Download protocol
FC protocol for CL594 Cathepsin L antibody CL594-66914Download protocol
Standard Protocols
Click here to view our Standard Protocols