CD68 Polyclonal antibody

CD68 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28058-1-AP

Synonyms

Scard1, gp110, CD68 antigen


☆大好評トライアルサイズ ¥20,000~! >>> 一次抗体特別価格キャンペーン開催中!


Tested Applications

Positive WB detected inRAW 264.7 cells, mouse spleen tissue, NR8383 cells
Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28058-1-AP targets CD68 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Cited Reactivitymouse, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD68 fusion protein Ag27827 相同性解析による交差性が予測される生物種
Full Name CD68 antigen
Calculated molecular weight 35 kDa
Observed molecular weight 100 kDa
GenBank accession numberNM_001291058
Gene symbol Cd68
Gene ID (NCBI) 12514
RRIDAB_2881049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
WB protocol for CD68 antibody 28058-1-APDownload protocol
IHC protocol for CD68 antibody 28058-1-APDownload protocol
IF protocol for CD68 antibody 28058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

Nature

Injectable ultrasonic sensor for wireless monitoring of intracranial signals

Authors - Hanchuan Tang
mouseIHC

Crit Care

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Authors - Lingbing Zhang
mouseIF

ACS Nano

Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition

Authors - Yanan Wang
mouseIF

J Pineal Res

Extracellular vesicles derived from melatonin-preconditioned mesenchymal stem cells containing USP29 repair traumatic spinal cord injury by stabilizing NRF2.

Authors - Wei Liu
mouseIF

J Clin Invest

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Authors - Xue Gong
IF

J Exp Clin Cancer Res

FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression

Authors - Wei-Min Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alexandru (Verified Customer) (11-13-2023)

Great antibody, worked well in my experiments.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Mouse ventricle
FH

Amelie (Verified Customer) (09-10-2019)

sections were deparaffinized and subjected to heat mediated antigen retrieval using TE buffer ph9On the pictures CD68 in red

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse adrenal gland
CD68 Antibody Immunofluorescence, validation (1:1000 dilution) in mouse adrenal gland (Cat no:28058-1-AP)