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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD68 Polyclonal antibody

CD68 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28058

Synonyms

Cd68, CD68 antigen, gp110, Scard1

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CoraLite® Plus 488-conjugated Cd68 Polyclonal anti (CL488-28058)

    IF Staining of mouse spleen using CL488-28058

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 Cd68 antibody (CL488-28058) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CoraLite® Plus 488-conjugated Cd68 Polyclonal anti (CL488-28058)

    IF Staining of mouse spleen using CL488-28058

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 Cd68 antibody (CL488-28058) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28058 targets CD68 in IF-P applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD68 fusion protein Ag27827 相同性解析による交差性が予測される生物種
Full Name CD68 antigen
Calculated molecular weight 35 kDa
Observed molecular weight 100 kDa
GenBank accession numberNM_001291058
Gene symbol Cd68
Gene ID (NCBI) 12514
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD68 antibody CL488-28058Download protocol
Standard Protocols
Click here to view our Standard Protocols