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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66156 targets Ceruloplasmin in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Ceruloplasmin fusion protein Ag15535 相同性解析による交差性が予測される生物種 |
| Full Name | ceruloplasmin (ferroxidase) |
| Calculated molecular weight | 1065 aa, 122 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC146663 |
| Gene Symbol | Ceruloplasmin |
| Gene ID (NCBI) | 1356 |
| RRID | AB_3084700 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00450 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Ceruloplasmin antibody CL594-66156 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |