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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Ch-TOG Monoclonal antibody

Ch-TOG Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H5G1

Cat no : CL594-67631

Synonyms

ch TOG, CHTOG, Ch-TOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Ch-TOG Monoclonal antibody (CL594-67631)

    IF Staining of HepG2 using CL594-67631

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Ch-TOG antibody (CL594-67631, Clone: 1H5G1 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67631 targets Ch-TOG in IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ch-TOG fusion protein Ag28770 相同性解析による交差性が予測される生物種
Full Name cytoskeleton associated protein 5
Observed molecular weight 220-240 kDa
GenBank accession numberBC120869
Gene symbol Ch-TOG
Gene ID (NCBI) 9793
RRIDAB_2920166
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

Protocols

Product Specific Protocols
IF protocol for CL594 Ch-TOG antibody CL594-67631Download protocol
Standard Protocols
Click here to view our Standard Protocols