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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using chicken Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

    100 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using chicken Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

    50 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using chicken Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

    25 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using chicken Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

    12.5 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using chicken Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

    12.5 nL chicken egg were subjected to SDS-PAGE under reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

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Chicken Chicken IgY Monoclonal antibody, PBS Only

Chicken IgY Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67405-1-PBS
Clone No.1E12G2

Host / Isotype

Mouse / IgG2a

Reactivity

Chicken

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67405-1-PBS targets Chicken IgY in WB, Indirect ELISA applications and shows reactivity with Chicken samples.

Tested Reactivity Chicken
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Chicken IgY
Observed molecular weight 180 kDa, 70 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2882646
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).