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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Chicken IgY Monoclonal antibody

Chicken IgY Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Chicken

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E12G2

Cat no : 67405-1-Ig

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Recombinant protein using Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig

    100 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

  • Western Blot (WB) analysis of Recombinant protein using Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig

    50 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

  • Western Blot (WB) analysis of Recombinant protein using Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig

    25 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

  • Western Blot (WB) analysis of Recombinant protein using Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig

    12.5 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

  • Western Blot (WB) analysis of Recombinant protein using Chicken IgY Monoclonal antibody (67405-1-Ig)

    WB analysis of Recombinant protein using 67405-1-Ig

    12.5 nL chicken egg were subjected to SDS-PAGE under reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inChicken IgY, Chicken egg

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67405-1-Ig targets Chicken IgY in WB, ELISA applications and shows reactivity with Chicken samples.

Tested Reactivity Chicken
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Chicken IgY
Observed molecular weight 180 kDa, 70 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_2882646
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).

Protocols

Product Specific Protocols
WB protocol for Chicken IgY antibody 67405-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Carbohydr Polym

Carboxymethyl chitosan assembled piezoelectric biosensor for rapid and label-free quantification of immunoglobulin Y.

Authors - Hao Li
View Article